Title page for ETD etd-11162007-064655


Type of Document Master's Thesis
Author Saenz, Jesse Ray
Author's Email Address jsaenz1@lsu.edu
URN etd-11162007-064655
Title Cryopreservation of White-Tail Deer Epididymal Sperm for Artificial Insemination
Degree Master of Science (M.S.)
Department Animal Science (Animal, Dairy, & Poultry Sciences)
Advisory Committee
Advisor Name Title
Robert A. Godke Committee Chair
Dale Paccamonti Committee Member
Kenneth R. Bondioli Committee Member
Keywords
  • White-tail deer
  • epididymal sperm
  • cryopreservation
  • artificial insemination
  • pregnancies
Date of Defense 2007-10-30
Availability unrestricted
Abstract
The ability to cryopreserve epididymal sperm from mature postmortem bucks has

long been of interest to both wildlife conservationists and deer ranchers. Increased

understanding of the cryobiology of epididymal sperm from a non domestic species,

such as White-tail deer, could aid in development of future protocols to assist in the

preservation of endangered species. In Experiment I, results showed that after cooling

postmortem bull testes for 22 hours, no significant difference was noted between sperm

parameters of epididymal sperm collected at room temperature or at a cool enviorment.

In Experiment II, it was shown that White-tail deer sperm could be successfully

cryopreserved using a bovine freezing protocol. Also, if immediate processing of

epididymal sperm is not an option, testes can be held within the scrotum at 10°C to 15°C

for up to 24 hours prior to processing the sperm for freezing. In Experiment III, post-thaw

normal sperm morphology results show that glycerol should be considered over DMSO

when freezing White-tail deer epididymal sperm. In Experiment IV, it was shown that

White-tail deer epididymal sperm can be held in the presence of glycerol for up to 12

hours and still result in post-thaw motility values >30%. Also, when comparing exposure

time of White-tail deer epididymal sperm to glycerol there was relatively no change in

post-thaw membrane integrity from 0 hours to 24 hours. In the final experiment, the

fawning rates of three different estrous synchronization protocols using timed artificial

insemination were compared over two consecutive breeding seasons. The most

preferred synchronization protocol was a 14 day CIDR with an eCG injection at the time

of CIDR removal. A preliminary experiment was then conducted using the 14 day CIDR

with eCG to synchronize and artificially inseminate six does with frozen-thawed

epididymal sperm, resulting in five healthy fawns from three pregnancies.

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