Type of Document Dissertation Author Albu, Sebastian Author's Email Address email@example.com URN etd-11122015-232818 Title Evolutionary Relationships among Fungal Soybean Pathogens and Molecular Marker Development in the Genus Cercospora Degree Doctor of Philosophy (Ph.D.) Department Plant Pathology & Crop Physiology Advisory Committee
Advisor Name Title Schneider, Raymond Committee Chair Doyle, Vinson Committee Co-Chair Aime, M. Catherine Committee Member Clark, Christopher Committee Member Cao, Quang Dean's Representative Keywords
Date of Defense 2015-10-28 Availability restricted AbstractCercospora leaf blight (CLB) and purple seed stain (PSS) are common soybean diseases in the Gulf South of the United States (USA). For nearly a century, Cercospora kikuchii has been considered as the only pathogen causing these diseases. However, previous reports of genetic diversity among isolates collected throughout Louisiana suggested the presence of multiple lineages or species. Recent systematic studies classified species of Cercospora using a taxonomic system based on phylogenetic analysis of five nuclear loci (legacy genes). Using a similar approach, cercosporoid fungi tentatively identified as C. kikuchii were evaluated along with 53 other species of Cercospora. No isolates from this study were nested within the clade including the ex-type strain of C. kikuchii. Five isolates grouped with C. cf. sigesbeckiae and all others were part of C. cf. flagellaris. Several isolates of C. cf. flagellaris were also obtained from Gossypyium hirsutum and Phytolacca americana. These results suggest that C. kikuchii is not the organism responsible for causing CLB or PSS in the Gulf South and other areas of the USA.
Multiple haplotypes were observed at each locus and individual genes varied in their resolving power. Most species were monophyletic in concatenated analyses, but reciprocal monophyly was generally not observed within individual gene trees. Furthermore, node support values were generally low across all topologies, indicating that the phylogenetic markers most commonly used for systematic studies of Cercospora are limited to answering shallow-level taxonomic questions. However, existing genome sequence data provided an excellent opportunity to develop new markers with stronger phylogenetic signal to better understand the evolutionary history of Cercospora. Sixty-three exon-flanked intergenic regions, syntenic between the genomes of C. cf sigesbeckiae and C. canescens, were extracted and aligned, then ranked and filtered according to several metrics to assess their phylogenetic utility. Candidate markers were validated by PCR on 24 Cercospora species, including 16 type strains. Assessment of phylogenetic informativeness profiles and phylogenetic analyses showed that all of the new markers provide greater interspecific resolution than the legacy genes and offer new options for identifying cryptic species in complex clades like C. cf. flagellaris.
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