Title page for ETD etd-1112103-071121

Type of Document Dissertation
Author Paul, Julius Braden
URN etd-1112103-071121
Title Proportional Assessment of X and Y Chromosome-Bearing Spermatozoa in Bull and Boar Ejaculates Using Conventional and Real-Time PCR Techniques
Degree Doctor of Philosophy (Ph.D.)
Department Dairy Science (Animal, Dairy, & Poultry Sciences)
Advisory Committee
Advisor Name Title
John E. Chandler Committee Chair
Donald L. Thompson Committee Member
Richard K. Cooper Committee Member
Sue G. Bartlett Committee Member
Shulin Li Dean's Representative
  • sex ratio
  • real-time PCR
  • Y chromosome
  • spermatozoa
  • boar
  • bull
Date of Defense 2003-10-31
Availability unrestricted
Considerable variation in the percentage of Y-chromosome bearing spermatozoa

(%Y-CBS) between ejaculates in the same male has been reported. Collection regime has

been speculated to influence the degree of variation in %Y-CBS in bull ejaculates

(Chandler et al., 1998). Experiments were designed to evaluate the effects of two

collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis

and image analysis was employed to determine %Y-CBS in ejaculates from two bulls

collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology

was used to quantify %Y-CBS in the same ejaculates. Boar ejaculates were also analyzed

with both techniques and compared to the percent male piglets in litters resulting from the

assayed ejaculates. Collection day (P<0.0001) significantly affected %Y-CBS as

determined by both PCR methods. Ejaculate nested within bull (P<0.07) was significant in

the conventional PCR study and collection frequency (P<0.0001) in the realtime PCR trial.

Ejaculate nested within boar was highly significant (P<0.0001) for both technologies.

Boar was significant (P<0.002) in the conventional PCR study. Predicted %Y-CBS

determined by real-time PCR was significantly correlated (0.52, P=0.004) to percent male

piglets. Both PCR techniques were effective in quantifying the variation in %Y-CBS in

bull and boar ejaculates. Manipulation of %Y-CBS via collection regime may prove

valuable for altering the secondary sex ratio in animal agriculture.

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