Title page for ETD etd-1112102-211702

Type of Document Dissertation
Author Collins II, Michael Gerard
Author's Email Address mcolli9@lsu.edu
URN etd-1112102-211702
Title Hypoxanthine Phosphoribosyl Transferase and Early Embryo Development
Degree Doctor of Philosophy (Ph.D.)
Department Animal Science (Animal, Dairy, and Poultry Sciences)
Advisory Committee
Advisor Name Title
Robert A. Godke Committee Chair
Dale L. Paccamonti Committee Member
J. Marcos Fernandez Committee Member
John W. Lynn Committee Member
Gary E. Wise Dean's Representative
  • hypoxanthine
  • hprt
  • embryo
  • transgenic
  • development
  • sex
Date of Defense 2002-04-30
Availability unrestricted
The objectives of the first experiment were to ascertain if there were any differences between two mouse areola and adipose tissue cell lines. These cell lines differed only in the presence or absence of the hypoxanthine phosphoribosyl transferase (HPRT) gene. It was found that the cell lines were similar for all parameters.

The objectives of the second experiment were to determine if the HPRT gene had an effect on the development of preimplantation mouse embryo. To test this transgenic mouse lines were created. The embryo sex and genotype ratio, the embryo diameter, and the embryo developmental patterns, using a Collin-Ryan Embryo Development (C-RED) score, were determined. The sex and genotype ratios differed from the expected 1:1. The experiment concluded that HPRT is effecting embryo development and sex ratios

The objectives of the third experiment were to determine if there is an effect of sex and HPRT on the cell number of mouse blastocysts. It was found that there was not an effect of sex, but that HPRT independent of sex was effecting the cell number of mouse blastocysts.

The objectives of the fourth experiments were to determine if the HPRT gene in co-culture would have an effect on the development and cell number of mouse blastocysts. The blastocysts co-cultured with cells containing the HPRT gene had significantly greater number of blastomeres. This experiment concluded that HPRT in co-culture can alter the cell number of mouse blastomeres.

The objective of the fifth experiment was to determine if the same results that were seen with the HPRT gene in co-culture on mouse embryos could be found with bovine embryos. There was significantly more blastocyst from co-culture with the cells containing the HPRT gene. There was not a difference in cell number of the bovine blastocyst.

The sixth experiment determined the effect of HPRT inhibitors and an inducer on bovine embryo development and sex ratios. It was found that the embryos developed with the inducer in the medium did not have altered sex ratios.

The results of these experiments demonstrate that HPRT is effecting mammalian embryo development.

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