| Type of Document |
Dissertation |
| Author |
Olsen, Philip Dmitri
|
| Author's Email Address |
peolsen@mindspring.com |
| URN |
etd-1111101-194018 |
| Title |
The Synthesis of Deuterated Arylamine DNA Adducts for Use in the Development of an Isotope Dilution LC/MS/MS Method |
| Degree |
Doctor of Philosophy (Ph.D.) |
| Department |
Chemistry |
| Advisory Committee |
| Advisor Name |
Title |
| Jay Means |
Committee Chair |
| Mark McLaughlin |
Committee Member |
| Steve Soper |
Committee Member |
| William Crowe |
Committee Member |
| Grover Waldrop |
Dean's Representative |
|
| Keywords |
- aromatic amines
- 2-aminofluorene
- benzidine
- DNA adducts
- mass spectrometry
|
| Date of Defense |
2001-09-18 |
| Availability |
unrestricted |
Abstract
Polycyclic aromatic amines (arylamines) are a class of chemical carcinogens that are prevalent in environmental and industrial settings. In order to study the mechanism of their toxicity a quantitative and qualitative detection method was developed to measure the C8-adenine adducts of benzidine and 2-aminofluorene in DNA samples. To do this a novel synthetic method using a palladium catalyst was developed to prepare authentic and deuterated arylamine adducts to serve as standards. These standards were then used to develop a high performance liquid chromatography, electrospray, tandem mass spectrometry, isotope dilution, detection method. To demonstrate the validity of this method two spike and recovery experiments were performed.
|
| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
|
| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
| |
Olsen_dis.pdf |
2.48 Mb |
00:11:29 |
00:05:54 |
00:05:10 |
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