Title page for ETD etd-1111101-194018


Type of Document Dissertation
Author Olsen, Philip Dmitri
Author's Email Address peolsen@mindspring.com
URN etd-1111101-194018
Title The Synthesis of Deuterated Arylamine DNA Adducts for Use in the Development of an Isotope Dilution LC/MS/MS Method
Degree Doctor of Philosophy (Ph.D.)
Department Chemistry
Advisory Committee
Advisor Name Title
Jay Means Committee Chair
Mark McLaughlin Committee Member
Steve Soper Committee Member
William Crowe Committee Member
Grover Waldrop Dean's Representative
Keywords
  • aromatic amines
  • 2-aminofluorene
  • benzidine
  • DNA adducts
  • mass spectrometry
Date of Defense 2001-09-18
Availability unrestricted
Abstract
Polycyclic aromatic amines (arylamines) are a class of chemical carcinogens that are prevalent in environmental and industrial settings. In order to study the mechanism of their toxicity a quantitative and qualitative detection method was developed to measure the C8-adenine adducts of benzidine and 2-aminofluorene in DNA samples. To do this a novel synthetic method using a palladium catalyst was developed to prepare authentic and deuterated arylamine adducts to serve as standards. These standards were then used to develop a high performance liquid chromatography, electrospray, tandem mass spectrometry, isotope dilution, detection method. To demonstrate the validity of this method two spike and recovery experiments were performed.
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