Type of Document Master's Thesis Author Carvajal-Aldaz, Diana Gabriela Author's Email Address email@example.com URN etd-11092012-160754 Title Inhibition of Adipocyte Differentiation in 3T3-L1 Cell Line by Quercetin or Isorhamnetin. Degree Master of Science (M.S.) Department Food Science Advisory Committee
Advisor Name Title Losso, Jack Committee Chair Enright, Frederick Committee Member Finley, John Committee Member Keywords
- adipocyte differentiation
Date of Defense 2012-11-08 Availability unrestricted AbstractCarvajal-Aldaz, Diana Gabriela, B.S. Food Science, Zamorano University, 2007
Master of Science, Fall Commencement, 2012
Major: Food Science
Inhibition of Adipocyte Differentiation in 3T3-L1 Cell Line by Quercetin or Isorhamnetin
Thesis directed by Professor Jack N. Losso
Pages in thesis, 43. Words in abstract, 281.
Obesity has become a major health problem worldwide. Obesity increases the risk of hypertension, diabetes, and certain types of cancer. Quercetin is a bioactive compound widely found in a variety of foods that are consumed daily. Isorhamnetin is a bioactive compound found in some foods and also is a quercetin metabolite. Many studies have reported the anti-obesity and anti-inflammation properties of quercetin and isorhamnetin. The objective of this study was to test the effect of quercetin or isorhamnetin at physiological and supraphysiological concentrations on the inhibition of the differentiation process of 3T3-L1 pre-adipocyte to adipocyte. Cell viability results demonstrated no significant difference (P > 0.05) between non differentiated cells, control and quercetin or isorhamnetin treated cells. During adipocyte differentiation for 8 days in the presence of quercetin or isorhamnetin, cell viability was above 94.84% and 97.63%, respectively. Red oil O staining assay was performed in order to evaluate the inhibitory effect of quercetin or isorhamnetin on cytoplasmic lipid droplet accumulation. Significant differences (P < 0.05) were reported. Isorhamnetin was more effective than quercetin in inhibiting cytoplasmic lipid droplet accumulation. Neither quercetin nor isorhamnetin had an effect on the expression of macrophage chemoattractant protein -1 (MCP-1). CCAAT/enhancer binding protein α (C/EBP-α) was down-regulated by quercetin or isorhamnetin. Compared to control quercetin decreased PPAR-γ 1 and 2 expressions by 45.03 ± 3.17% and 27.58 ± 12.39%, while isorhamnetin decreased PPAR-γ 1 and 2 expressions by 41.48 ± 9.51% and 2.01 ± 32.46%, respectively. β-catenin was not dose dependent either for quercetin or isorhamnetin and did not follow a specific trend. Taken together, our data indicate that isorhamnetin more than quercetin can exert potential anti-obesity effects by inhibiting differentiation of pre-adipocytes at physiological concentrations.
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