Title page for ETD etd-11092012-160754


Type of Document Master's Thesis
Author Carvajal-Aldaz, Diana Gabriela
Author's Email Address dcarva1@tigers.lsu.edu
URN etd-11092012-160754
Title Inhibition of Adipocyte Differentiation in 3T3-L1 Cell Line by Quercetin or Isorhamnetin.
Degree Master of Science (M.S.)
Department Food Science
Advisory Committee
Advisor Name Title
Losso, Jack Committee Chair
Enright, Frederick Committee Member
Finley, John Committee Member
Keywords
  • 3T3-L1
  • quercetin
  • inhibition
  • isorhamnetin
  • adipocyte differentiation
Date of Defense 2012-11-08
Availability unrestricted
Abstract
Carvajal-Aldaz, Diana Gabriela, B.S. Food Science, Zamorano University, 2007

Master of Science, Fall Commencement, 2012

Major: Food Science

Inhibition of Adipocyte Differentiation in 3T3-L1 Cell Line by Quercetin or Isorhamnetin

Thesis directed by Professor Jack N. Losso

Pages in thesis, 43. Words in abstract, 281.

ABSTRACT

Obesity has become a major health problem worldwide. Obesity increases the risk of hypertension, diabetes, and certain types of cancer. Quercetin is a bioactive compound widely found in a variety of foods that are consumed daily. Isorhamnetin is a bioactive compound found in some foods and also is a quercetin metabolite. Many studies have reported the anti-obesity and anti-inflammation properties of quercetin and isorhamnetin. The objective of this study was to test the effect of quercetin or isorhamnetin at physiological and supraphysiological concentrations on the inhibition of the differentiation process of 3T3-L1 pre-adipocyte to adipocyte. Cell viability results demonstrated no significant difference (P > 0.05) between non differentiated cells, control and quercetin or isorhamnetin treated cells. During adipocyte differentiation for 8 days in the presence of quercetin or isorhamnetin, cell viability was above 94.84% and 97.63%, respectively. Red oil O staining assay was performed in order to evaluate the inhibitory effect of quercetin or isorhamnetin on cytoplasmic lipid droplet accumulation. Significant differences (P < 0.05) were reported. Isorhamnetin was more effective than quercetin in inhibiting cytoplasmic lipid droplet accumulation. Neither quercetin nor isorhamnetin had an effect on the expression of macrophage chemoattractant protein -1 (MCP-1). CCAAT/enhancer binding protein α (C/EBP-α) was down-regulated by quercetin or isorhamnetin. Compared to control quercetin decreased PPAR-γ 1 and 2 expressions by 45.03 3.17% and 27.58 12.39%, while isorhamnetin decreased PPAR-γ 1 and 2 expressions by 41.48 9.51% and 2.01 32.46%, respectively. β-catenin was not dose dependent either for quercetin or isorhamnetin and did not follow a specific trend. Taken together, our data indicate that isorhamnetin more than quercetin can exert potential anti-obesity effects by inhibiting differentiation of pre-adipocytes at physiological concentrations.

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