Title page for ETD etd-0902103-150730

Type of Document Master's Thesis
Author Beem, Amy Elizabeth
Author's Email Address abeem1@lsu.edu
URN etd-0902103-150730
Title Use of Urine PH to Predict Incidence of Ketosis in Transition Dairy Cows
Degree Master of Science (M.S.)
Department Animal Science (Animal, Dairy, & Poultry Sciences)
Advisory Committee
Advisor Name Title
H. Gale Bateman Committee Chair
Bruce Jenny Committee Member
Cathy Williams Committee Member
Charles Hutchinson Committee Member
  • ketosis
  • transition
  • urine ph
Date of Defense 2003-08-05
Availability unrestricted
Forty-one (10 primiparous and 31 multiparous) Holstein cows were used in an experiment to determine the effects of prepartum dietary energy (PDE) and supplemental Ca-propionate on the incidence of ketosis in transition dairy cows and to evaluate the usefulness of urine pH as a predictor for ketosis. Treatment factors were prepartum dietary energy concentration (normal vs. high) with or without 113.5g Ca-Propionate/day (Nutro CalŪ, Kemin Industries, Inc., Des Moines, IA). All cows were fed the same basal diet postpartum. Ca-propionate supplementation continued postpartum. Cows were individually fed and dry matter intakes (DMI) were measured daily. Milk production was measured and sampled at each milking for 3 weeks postpartum and analyzed for % fat, % protein, and SCC (somatic cell count). Blood samples were collected prior to the afternoon milking 3x/ wk and analyzed for β-hydroxybutyrate (BHBA) and glucose concentrations. Urine was collected 3x/ wk and analyzed for pH, acetoacetate, and BHBA. Treatment did not affect mean DMI, milk yield, milk component production or percentage, 4% FCM, SCCS, BWT, urine pH, urine acetoacetate, urine BHBA, plasma BHBA, or plasma glucose. There was a statistical interaction of Ca-propionate supplementation and week for urine pH and BHBA. Correlation coefficients between urine BHBA, urine pH, and urine acetoacetate were not useful for prediction of ketosis across all cows. However, they were numerically higher when restricted to data from an individual cow. Correlation coefficients between BHBA and acetoacetate concentrations in urine were significant. These data suggest that PDE and Ca-propionate supplementation had no effect on the incidence of ketosis, however Ca-propionate supplementation allowed cows to recover faster from ketosis postpartum. The use of urine pH was not a useful tool for the prediction of ketosis across a herd, but may be a useful indicator of ketosis when compared within an individual animal. Due to factors unrelated to treatment diets, there was a high incidence of health disorders such as retained fetal membranes, metritis, displaced abomasums, and laminitis were observed during this trial that probably affected any observed treatment responses.
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