Title page for ETD etd-09012006-091730


Type of Document Master's Thesis
Author Chapman, Anna Marie
Author's Email Address achapman@mail.vetmed.lsu.edu
URN etd-09012006-091730
Title Characterizing Salmonella Fecal Shedding among Racehorses in Louisiana
Degree Master of Science (M.S.)
Department Veterinary Clinical Sciences
Advisory Committee
Advisor Name Title
Susan C. Eades Committee Chair
Almay F. Roy Committee Member
Jill Johnson Committee Member
Mark A. Mitchell Committee Member
Keywords
  • salmonella
  • horse
  • culture
  • polymerase chain reaction
  • delayed secondary enrichment
Date of Defense 2006-05-17
Availability unrestricted
Abstract
Salmonella is an important intestinal pathogen in horses capable of infecting populations without demonstrating clinical illness. This study was performed to determine the prevalence of Salmonella fecal shedding among racehorses in Louisiana. Three serial fecal samples were collected from 429 Thoroughbred horses housed at four racetracks. Feces were tested for Salmonella by microbiologic culture with selective primary enrichment and delayed secondary enrichment (DSE). Samples were also evaluated for the presence of Salmonella by polymerase chain reaction (PCR) using genus-specific oligonucleotide primers. A total of 7 (1.6%) horses were positive for Salmonella by either primary bacterial culture or DSE and an additional 2 horses (0.5%) were positive for Salmonella by PCR. The combined prevalence of Salmonella fecal shedding from among all the horses in this study was 2.1%. The results of this study suggest that the prevalence of fecal shedding of Salmonella among racehorses in Louisiana is low.
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