Title page for ETD etd-0820102-120526

Type of Document Master's Thesis
Author Badal, Rohit
Author's Email Address rbadal1@lsu.edu
URN etd-0820102-120526
Title Supercritical Carbon Dioxide Extraction of Lipids from Raw and Bioconverted Rice Bran
Degree Master of Science in Biological & Agricultural Engineering (M.S.B.A.E.)
Department Biological and Agricultural Engineering
Advisory Committee
Advisor Name Title
Terry Walker Committee Chair
Caye M. Drapcho Committee Member
J. Samuel Godber Committee Member
  • oil
  • sfe
Date of Defense 2002-04-25
Availability unrestricted
Rice bran is often discarded despite its nutritional value because the oil in rice bran easily becomes rancid. This has necessitated the search for a method for utilizing the rice bran. The present study involves the application of supercritical fluid extraction (SFE) and bioprocessing (microbial growth on rice bran media) for increasing the value of the rice bran to produce products of high value (nutraceuticals) with superior health benefit. This study was focused on determining the effect of particle size (>48 mesh and 16-48 mesh) and biotreatment on the yield and quality of rice bran oil. SFE technique (40C, 4000 psi, 200 standard cm3 per min) was applied for extracting oil from raw and bioconverted rice bran and kinetic data was collected. Fatty acid methyl ester (FAME) analysis was accomplished with gas chromatography to determine fatty acid distribution in the raw and bioconverted rice bran. Statistical analysis was done to determine the effect of the parameters (particle size, biotreatment and time) on the oil yield and (FAME) composition. The results show SFE yielded 51.5% of the total ether extractable oil in 2 hours from small particle size rice bran. FAME analysis showed that Eicosapentaenoic acid, (1.8 FAME wt %) and Arachidonic acid (0.67 FAME wt %) produced during the biotreatment was extracted by SFE. The statistical analysis indicated oil yield was a function of particle size for SFE (p =0.0013), but not for Soxhlet extraction.
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