Title page for ETD etd-07272012-151912


Type of Document Master's Thesis
Author Terrell, Lekeith
URN etd-07272012-151912
Title Modulation of Mesenchymal Stem Cell Behavior by Nano and Micro-Sized Beta-Tricalcium Phosphate Particles in Suspension and Composite Structures
Degree Master of Science in Biological & Agricultural Engineering (M.S.B.A.E.)
Department Biological & Agricultural Engineering
Advisory Committee
Advisor Name Title
Daniel Hayes Committee Chair
Steven G. Hall Committee Member
W. Todd Monroe Committee Member
Keywords
  • composites
  • stem cell
  • plla
  • tricalcium phosphate
  • nano
Date of Defense 2012-04-12
Availability unrestricted
Abstract
Nano and micro particles have steadily grown in interest for synthetic bone composite structures. Nano and micro sized calcium phosphates especially are of interest because of the osteoinductive behavior of calcium phosphate. Engineered composites incorporating polymers and ceramics, such as poly l-lactic acid (PLLA)/β-TCP, for bone tissue regeneration have been well investigated for their proliferative and osteoinductive abilities. Limited research has been done to thoroughly investigate the effects of various sized β-TCP particles on human mesenchymal stem cell behavior. Foreign bodies are known to elicit an inflammatory and cytotoxic response and several nano-particles are known to elicit cytotoxic and pro-inflammatory responses of mesenchymal stem cells. In this study, the modulation of adult human adipose-derived mesenchymal stem cell (hASC) behavior exposed to β-TCP, as functions of particle size, content and composition in a polymeric-ceramic scaffold system was investigated. The objectives of this work include the development and characterization of monolithic 3-D polymeric-ceramic structures using a modified unidirectional fabrication technique to investigate the cytotoxic, pro-inflammatory and osteogenic potential of PLLA-β-TCP composite on adult human stem cells. Characterization of PLLA-β-TCP composites showed that increasing β-TCP content the compressive strength decreases. Viability of hASCs over a 7 day exposure study showed increasing cytotoxicity with respect to increasing particle concentration regardless of size, although acute (24hr) cytotoxicity was more influenced by size. qPCR was used to measure the pro-inflammatory (IL-6) expression as well as early osteogenic differentiation (hALP). Results suggest there is a modulation in stem cell behavior as a function of β-TCP particle size, concentration and composite system.
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