Title page for ETD etd-07122006-135145


Type of Document Dissertation
Author Xu, Yanlong
Author's Email Address yxu3@lsu.edu
URN etd-07122006-135145
Title Simulating Protein Evolution Via Thermodynamic Models
Degree Doctor of Philosophy (Ph.D.)
Department Chemistry
Advisory Committee
Advisor Name Title
Randall Hall Committee Chair
Bin Chen Committee Member
David Pollock Committee Member
Marcia Newcomer Committee Member
Giselle Hosgood Pagel Dean's Representative
Keywords
  • protein
  • evolution
  • model
  • thermodynamic
Date of Defense 2006-07-07
Availability unrestricted
Abstract
Natural proteins are results of evolution and they need to maintain certain thermodynamic stabilities in order to carry out their biological functions. By simulating protein evolution based on thermodynamic rules, we could reconstruct the evolution trajectory and analyze the evolutionary dynamics of a protein population, and further understand the protein sequence-structure-function relationship. In this study, we have used both a simplified lattice model and a high-resolution atomic model to simulate protein evolution processes. With the lattice model, we have investigated general theoretical questions about how protein structural designability would affect protein evolution, particularly how it would affect protein recombination and protein-ligand interactions in the evolution process. With the atomic model, we could simulate evolution processes for particular protein with different selection pressure. First, we simulated directed evolution processes and utilized such model to investigate the thermostabilization of T4 lysozyme. Second, we simulated neutral evolution processes for HIV protease, investigated its evolutionary dynamics and the possible drug-resistance mechanism in such neutral evolution. Overall, thermodynamic models can help us understand either general protein evolution dynamics or specific protein sequence-structure-function relationship in evolution.
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