Title page for ETD etd-07072004-114606

Type of Document Master's Thesis
Author Kolar, Praveen
Author's Email Address pkolar1@lsu.edu
URN etd-07072004-114606
Title Design and Development of a Process-Control Temperature System for Eastern Oyster Crassostrea Virginica Research
Degree Master of Science in Biological & Agricultural Engineering (M.S.B.A.E.)
Department Biological & Agricultural Engineering
Advisory Committee
Advisor Name Title
Steven G. Hall Committee Chair
Charles J. Monlezun Committee Member
Jerome F. La Peyre Committee Member
Terrence R. Tiersch Committee Member
  • process control
  • eastern oyster
  • temperature
Date of Defense 2004-05-20
Availability unrestricted
A process-control temperature system was designed and developed for facilitating somatic cell research on eastern oyster Crassostrea virginica. The system consisted of sixteen independent tanks capable of individual temperature regimes controlled by a central computer. Food grade 250-L tanks were used to hold 60 oysters per tank. Each tank was equipped with heating and cooling mechanisms. Immersion heaters were used for heating and a pump and a stainless steel heat exchangers immersed in chilled water (~ 7C) were used for cooling. Type-T thermocouples along with a CIOEXP32 multiplexer board (Measurement Computing Inc.) were used to measure water temperatures. Control was achieved via a pair of analog and digital (A/D) boards that actuated heaters and pumps through solid state relays.

The performance of the system was evaluated in two experiments for studies on somatic cell proliferation in eastern oysters. In the first experiment, the system was programmed to maintain constant temperatures of 10, 15, and 20C for a 20-day period. The system was able to maintain temperatures within 0.2C. In the second experiment, diurnal fluctuations of 20 1C, 20 5C, 15 1C, 15 5C, and 15 10C were imposed. The system was able to simulate water temperatures within 0.2C for regimes 20 1C, 20 5C, 15 1C.

A computer program Realwire for measuring and simulating real-time water temperatures was developed using freely available public domain software called Python and GNUwget. The software was able to measure and simulate real-time water temperatures.

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