Title page for ETD etd-0626103-112039


Type of Document Master's Thesis
Author Satterly, Neal Gilpin
Author's Email Address nsatte1@lsu.edu
URN etd-0626103-112039
Title PCR Inhibition and Toxic Effects by Sediment Samples Exposed to Drilling Muds
Degree Master of Science (M.S.)
Department Environmental Studies
Advisory Committee
Advisor Name Title
Paul LaRock Committee Co-Chair
Vince Wilson Committee Co-Chair
Ralph Portier Committee Member
Keywords
  • PCR Inhibition
  • sediment toxicity
  • drilling muds
Date of Defense 2003-06-24
Availability unrestricted
Abstract
This study evaluated the PCR inhibition and toxic effects by sediment samples exposed to drilling muds. This information was gathered in an attempt to identify what effect, if any, drilling muds had on the microbial community structure in sediments around four specific oil-drilling platforms in the Gulf of Mexico. Sediment samples were taken before and after drilling had taken place around two platforms (GB516 and VK916), and sediment samples were taken after drilling had taken place around two other platforms (MC292 and GB602). After using traditional, non-selective DNA extraction methods, successful amplification took place via PCR with all pre-drilling samples that were tested. Post-drilling samples, however, failed to amplify using the same procedure. Many experiments were conducted to identify the cause of PCR inhibition in the post-drilling samples. It was clearly demonstrated that an abundance of humic substances was being carried through the extraction procedure and caused the PCR inhibition. The Lumitox assay showed that the toxic effects of exposed sediments ranged from very toxic to non-toxic.
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