Title page for ETD etd-06172011-090919

Type of Document Master's Thesis
Author Sarjeant, Kelesha Crystal
Author's Email Address ksarje1@tigers.lsu.edu
URN etd-06172011-090919
Title The Expression of Cardiotrophin-1 Is Differentially Regulated in Murine and Human Obesity Type II Diabetes
Degree Master of Science (M.S.)
Department Biochemistry (Biological Sciences)
Advisory Committee
Advisor Name Title
Stephens, Jacqueline M. Committee Chair
Bartlett, Sue G. Committee Member
DiMario, Patrick Committee Member
  • 3T3-L1
  • insulin resistance
  • western blotting
Date of Defense 2011-06-08
Availability unrestricted
Cardiovascular disease is the leading cause of mortality in all developed nations. Several independent studies have shown that cardiotrophin-1 (CT-1) serum levels are modulated in patients with various types of cardiovascular disease including ischemic heart disease, valvular heart disease, and accelerated arthrosclerosis. CT-1 is a member of the Interleukin-6 family, or gp130 family of cytokines. It is also known to induce cardiomyocyte hypertrophy in vitro and in vivo, and is a critical component for cardiomyocyte survival. CT-1 is a naturally occurring protein with a molecular mass of approximately 21.5 kD and a 200 amino acid long sequence, it was discovered in a cDNA screen of murine stem cells and was originally identified in cardiomyocytes. Since then, CT-1 expression has been reported in several other tissues including skeletal muscle, liver, ovary, kidney and lung.

Interestingly, our studies reveal that CT-1 protein expression is significantly modulated in adipose tissue following high fat feeding in C57BL/6 mice. Of note, we did not observe regulation of CT-1 expression in white adipose tissue in human obesity and Type II Diabetes Mellitus (T2DM). We can readily detect CT-1 in the media of cultured adipocytes. This study suggests that CT-1 secretion from murine adipose tissue may be an important contributor to the levels of circulating CT-1. In summary, our studies demonstrate that CT-1 is an adipokine that is modulated in murine obesity and T2DM.

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