Type of Document Dissertation Author Park, Taehyun URN etd-04262011-153646 Title Small Footprint High Flow Rate Microdevice for Rare Target Cell Capture Degree Doctor of Philosophy (Ph.D.) Department Mechanical Engineering Advisory Committee
Advisor Name Title Murphy, Michael C. Committee Chair Choi, Jinwoo Committee Member Devireddy, Ramachandra Committee Member Nikitopoulos, Dimitris Committee Member Soper, Steven A. Committee Member Monroe, William Todd Dean's Representative Keywords
- Microdevice
- MEMS
- BioMEMS
- Cancer Diagnosis
- Circulating Tumor Cell
- CTC
- High Flow Rate
Date of Defense 2011-04-14 Availability unrestricted Abstract A novel high flow rate cell capture design was introduced to overcome the limitations of the current technologies or methods for rare target cell capture. Even though the rare target cell capture using BioMEMS technology has great potential for cancer diagnosis, previous rare cell capture research could not overcome the limitations of low flow rate or low recovery rate.Rare cell research requires precise sample preparation for accurate results. A new method of preparation for a single or a precise number of target cell was introduced. Current sample preparation methods which are not suitable for rare cell research, such as CTC capture or single cell analysis do not provide precise cell counts below 100. A cell collection chip was designed and used with a polyimide removed capillary tube to collect an exact number of target cells under a microscope.
To optimize the dimensions of the high flow rate design, CFD (Fluent v6.3, ANSYS, Inc., Canonsburg, PA, USA) simulation was used. The design focused on a high flow rate at inlet and low axial and lateral velocities in the cell capture regions with a small footprint. Based on the simulation results, the dimensions for several prototypes were determined and fabricated in PMMA.
The CTCs, MCF-7 cells, were captured flow rates up to 750 µL/min from 40% red blood cells with 80% recovery rate using the high flow rate device.
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