Title page for ETD etd-04112008-083508

Type of Document Master's Thesis
Author Sanchez-Migallon Guzman, David
Author's Email Address dguzman@vetmed.lsu.edu
URN etd-04112008-083508
Title Randomized Controlled Trial Evaluating Adeno-MOMP and MOMP DNA Vaccines Against Chlamydophila psittaci Challenge in Cockatiels (Nymphicus hollandicus)
Degree Master of Science (M.S.)
Department Veterinary Clinical Sciences
Advisory Committee
Advisor Name Title
Thomas N Tully Committee Chair
Abolghasem Baghian Committee Member
Branson Ritchie Committee Member
Mark A Mitchell Committee Member
Date of Defense 2008-03-14
Availability unrestricted
Chlamydophila psittaci causes severe disease in birds and humans, and important economical

losses in the avian companion and poultry industry. Vaccines are the most cost-effective

measure to control and help prevent infectious diseases, but to date there is no commercial

vaccine available. A randomized clinical trial was conducted to assess the efficacy of two

recombinant DNA vaccines against C. psittaci in Cockatiels (Nymphicus hollandicus). The first

recombinant DNA vaccine has a gene encoding MOMP and the immunostimulant chitosan

(MOMP DNA vaccine). The second recombinant DNA vaccine, contained the gene encoding

MOMP that was vectored by a replication defective human adenovirus (adeno-MOMP vaccine).

Forty adult cockatiels (Nymphicus hollandicus) were used for this study, and divided into each of

the vaccinated groups (n=10), positive control (n=10) and a negative control (n=10). The animals

were vaccinated on days 0 and 21 with the corresponding vaccine (DNA MOMP vaccine group,

adeno-MOMP vaccine group, negative control group) or placebo (positive control group), and

both vaccine groups and the positive control were challenged on day 42 Receiving 0.1 ml of

inoculum IN containing approximately 106 C. psittaci live organisms. The negative control group

was not challenged with any live organisms. The animals were monitored daily for the presence

of rhinitis, conjunctivitis, dyspnea, diarrhea and depression. On days 46, 49, 52, 55, 70 and 82,

combined choanal and cloacal swabs were taken and submitted for C. psittaci PCR. The

surviving birds were tested on day 82 for antichlamydial antibodies (IFA) and for the presence of

C. psittaci (PCR) from whole blood and combined choanal-cloacal swabs, and then humanely

euthanized. The birds were submitted for necropsy and examined for the presence of

macroscopic lesions on conjunctiva, lungs, airsacs, heart, spleen and liver. Individual samples from each of those tissues were taken for histopathology and pooled samples were submitted for C. psittaci culture.

There was a failure to detect antibody response by indirect immunofluorescent assay. The

cockatiels developed mild clinical signs and minimal mortality after challenge. The necropsy

and histopathologic evaluation of the tissues revealed mild to moderate lesions and no

significant difference with positive control. Further studies are needed to evaluate the efficacy of the vaccines.

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