Type of Document Master's Thesis Author Sanchez-Migallon Guzman, David Author's Email Address dguzman@vetmed.lsu.edu URN etd-04112008-083508 Title Randomized Controlled Trial Evaluating Adeno-MOMP and MOMP DNA Vaccines Against Chlamydophila psittaci Challenge in Cockatiels (Nymphicus hollandicus) Degree Master of Science (M.S.) Department Veterinary Clinical Sciences Advisory Committee
Advisor Name Title Thomas N Tully Committee Chair Abolghasem Baghian Committee Member Branson Ritchie Committee Member Mark A Mitchell Committee Member Keywords
- VACCINE CHLAMYDOPHILA PSITTACI CHALLENGE COCKATIE
Date of Defense 2008-03-14 Availability unrestricted Abstract Chlamydophila psittaci causes severe disease in birds and humans, and important economicallosses in the avian companion and poultry industry. Vaccines are the most cost-effective
measure to control and help prevent infectious diseases, but to date there is no commercial
vaccine available. A randomized clinical trial was conducted to assess the efficacy of two
recombinant DNA vaccines against C. psittaci in Cockatiels (Nymphicus hollandicus). The first
recombinant DNA vaccine has a gene encoding MOMP and the immunostimulant chitosan
(MOMP DNA vaccine). The second recombinant DNA vaccine, contained the gene encoding
MOMP that was vectored by a replication defective human adenovirus (adeno-MOMP vaccine).
Forty adult cockatiels (Nymphicus hollandicus) were used for this study, and divided into each of
the vaccinated groups (n=10), positive control (n=10) and a negative control (n=10). The animals
were vaccinated on days 0 and 21 with the corresponding vaccine (DNA MOMP vaccine group,
adeno-MOMP vaccine group, negative control group) or placebo (positive control group), and
both vaccine groups and the positive control were challenged on day 42 Receiving 0.1 ml of
inoculum IN containing approximately 106 C. psittaci live organisms. The negative control group
was not challenged with any live organisms. The animals were monitored daily for the presence
of rhinitis, conjunctivitis, dyspnea, diarrhea and depression. On days 46, 49, 52, 55, 70 and 82,
combined choanal and cloacal swabs were taken and submitted for C. psittaci PCR. The
surviving birds were tested on day 82 for antichlamydial antibodies (IFA) and for the presence of
C. psittaci (PCR) from whole blood and combined choanal-cloacal swabs, and then humanely
euthanized. The birds were submitted for necropsy and examined for the presence of
macroscopic lesions on conjunctiva, lungs, airsacs, heart, spleen and liver. Individual samples from each of those tissues were taken for histopathology and pooled samples were submitted for C. psittaci culture.
There was a failure to detect antibody response by indirect immunofluorescent assay. The
cockatiels developed mild clinical signs and minimal mortality after challenge. The necropsy
and histopathologic evaluation of the tissues revealed mild to moderate lesions and no
significant difference with positive control. Further studies are needed to evaluate the efficacy of the vaccines.
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