| Type of Document |
Dissertation |
| Author |
Alpturk, Onur
|
| Author's Email Address |
oalptu1@lsu.edu |
| URN |
etd-04112007-144751 |
| Title |
Lanthanide Complexes as
Fluorescent Indicators for Neutral Sugars and Cancer Biomarkers |
| Degree |
Doctor of Philosophy (Ph.D.) |
| Department |
Chemistry |
| Advisory Committee |
| Advisor Name |
Title |
| Robert Strongin |
Committee Chair |
| Carol Taylor |
Committee Member |
| David Spivak |
Committee Member |
| William Crowe |
Committee Member |
| Ilya Vekhter |
Dean's Representative |
|
| Keywords |
- lanthanides
- chemosensor
- biomarkers
|
| Date of Defense |
2007-03-20 |
| Availability |
unrestricted |
Abstract
Simple water soluble lanthanum and europium complexes are effective at detecting neutral sugars as well as glyco- and phospholipids. In solutions at physiologically relevant pH the fluorescent lanthanum complex binds neutral sugars with apparent binding constants comparable to those of arylboronic acids. Interference from commonly occurring anions is minimal. The europium complex detects sialic acid-containing gangliosides at pH 7.0 over an asialoganglioside. This selectivity is attributed, in large part, to the cooperative complexation of the oligosaccharide and sialic acid residues to the metal center, based on analogous prior studies. In MeOH, lysophosphatidic acid (LPA), a biomarker for several pathological conditions including ovarian cancer, is selectively detected by the europium complex. LPA is also detected via a fluorescence increase in human plasma samples. The 2-sn-OH moiety of LPA plays a key role in promoting binding to the metal center. Other molecules found in common brain ganglioside and phospholipid extracts do not interfere in the ganglioside or LPA fluorescence assays.
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| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
|
| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
| |
Full_Thesis.pdf |
2.20 Mb |
00:10:10 |
00:05:13 |
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