Title page for ETD etd-04112007-144751

Type of Document Dissertation
Author Alpturk, Onur
Author's Email Address oalptu1@lsu.edu
URN etd-04112007-144751
Title Lanthanide Complexes as Fluorescent Indicators for Neutral Sugars and Cancer Biomarkers
Degree Doctor of Philosophy (Ph.D.)
Department Chemistry
Advisory Committee
Advisor Name Title
Robert Strongin Committee Chair
Carol Taylor Committee Member
David Spivak Committee Member
William Crowe Committee Member
Ilya Vekhter Dean's Representative
  • lanthanides
  • chemosensor
  • biomarkers
Date of Defense 2007-03-20
Availability unrestricted
Simple water soluble lanthanum and europium complexes are effective at detecting neutral sugars as well as glyco- and phospholipids. In solutions at physiologically relevant pH the fluorescent lanthanum complex binds neutral sugars with apparent binding constants comparable to those of arylboronic acids. Interference from commonly occurring anions is minimal. The europium complex detects sialic acid-containing gangliosides at pH 7.0 over an asialoganglioside. This selectivity is attributed, in large part, to the cooperative complexation of the oligosaccharide and sialic acid residues to the metal center, based on analogous prior studies. In MeOH, lysophosphatidic acid (LPA), a biomarker for several pathological conditions including ovarian cancer, is selectively detected by the europium complex. LPA is also detected via a fluorescence increase in human plasma samples. The 2-sn-OH moiety of LPA plays a key role in promoting binding to the metal center. Other molecules found in common brain ganglioside and phospholipid extracts do not interfere in the ganglioside or LPA fluorescence assays.

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