Title page for ETD etd-03022007-141419


Type of Document Master's Thesis
Author Briere, Catherine Alix
Author's Email Address cbriere@vetmed.lsu.edu
URN etd-03022007-141419
Title Carprofen Compromises the Integrity and Barrier Function of the Colonic Mucosa of the Dog
Degree Master of Science (M.S.)
Department Veterinary Clinical Sciences
Advisory Committee
Advisor Name Title
Giselle Hosgood Committee Chair
Cheryl S. Hedlund Committee Member
Rebecca S. McConnico Committee Member
Keywords
  • gastrointestinal
Date of Defense 2007-01-24
Availability unrestricted
Abstract
Effects of carprofen on colon of dog have not been investigated.

Objectives

1) Measure conductance and permeability to mannitol of transverse, proximal descending and distal descending colonic mucosa of dog.

2) Measure conductance and permeability to mannitol of colonic mucosa of dog in presence of carprofen.

Design

In vitro experimental - nested, randomized block design

Animals

Colonic mucosa from 6 (objective 1) and 7 (objective 2) mature mixed-breed dogs.

Methods

Objective 1) Control - Three sections of mucosa from each region of colon were mounted in Ussing chamber units. Conductance was calculated every 15 min for 240 min. Flux of mannitol was calculated for three periods of one hour.

Objective 2) Carprofen - Methods based on results for objective 1. Sections of mucosa were prepared as in objective 1. Carprofen (400μg/ml) was added to bathing solution. Data for conductance and flux of mannitol was obtained as in objective 1.

Histologic examination of all sections was performed after experiment.

For both objectives, conductance was graphed against time for each chamber and area under each curve calculated. Conductance*time, flux of mannitol and frequency distribution of histologic categories were used for analysis.

Results

Objective 1) Mean +/- SEM conductance*time transverse colonic mucosa was higher than proximal and distal descending. Mean +/- SEM flux of mannitol increased from period 1 to period 3 for transverse colonic mucosa. Objective 2) Data from objective 1 was used as control for objective 2. Mean +/- SEM conductance*time carprofen group was higher than control group for all regions of colon. For carprofen group, mean +/- SEM flux of mannitol increased from period 1 to period 2 and from period 2 to period 3 for all regions of colon. There was higher proportion of sections with severe sloughing of cells and erosions involving more than 10% of epithelium in carprofen group compared to control.

Conclusion

Carprofen increases in vitro conductance and permeability to mannitol and causes sloughing of cells and erosions of colonic mucosa of dog which suggests compromise of integrity and loss of barrier function.

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