Title page for ETD etd-01082005-103926


Type of Document Master's Thesis
Author Ferrer, Marí­a Soledad
Author's Email Address mferrer@vetmed.lsu.edu
URN etd-01082005-103926
Title Post-Breeding Endometritis after Low Dose Insemination in the Mare
Degree Master of Science (M.S.)
Department Veterinary Clinical Sciences (Veterinary Medical Sciences)
Advisory Committee
Advisor Name Title
Dale Paccamonti Committee Chair
Bruce Eilts Committee Member
Robert Godke Committee Member
Keywords
  • endometritis
  • mare
  • hysteroscopic insemination
  • low-dose insemination
  • delayed uterine clearance
Date of Defense 2004-11-30
Availability unrestricted
Abstract
Hysteroscopic insemination in mares with delayed uterine clearance (DUC mares) is controversial. While some authors proposed that insemination with a reduced volume and number of spermatozoa may reduce post-mating endometritis, others proposed that the hysteroscopic procedure is inflammatory and should not be used in DUC mares. The overall objectives of this study were to evaluate the incidence and severity of post-mating endometritis in reproductively normal and DUC mares after hysteroscopic insemination at the uterotubal junction, and to determine if hysteroscopic insemination could be used in DUC mares to reduce post-mating endometritis. The mares were classified as normal or DUC based on the presence of intrauterine fluid 24 or 48 hours after a semen challenge. In Experiment 1, the acute endometritis was evaluated 24 hours after insemination. Each mare (n=5 normal, n=5 DUC) received three treatments in three consecutive estrous cycles: UB: uterine body insemination (one billion spermatozoa, 20 mL), HYST: hysteroscopic insemination (five million spermatozoa, 0.5 mL) and SHAM: sham hysteroscopic insemination (semen extender, 0.5 mL). Intrauterine fluid accumulation and uterine leukocyte numbers were not influenced by treatment. In the second experiment, residual endometritis was assessed 48 hours after insemination, and the effect of insemination method on fertility was evaluated. Each mare (n=4 normal, n=5 DUC) received four treatments in four consecutive estrous cycles: UB: uterine body insemination (one billion spermatozoa, 20 mL), HYST: hysteroscopic insemination (five million spermatozoa, 0.5 mL), SHAM: sham hysteroscopic insemination (semen extender, 0.5 mL) and SP: hysteroscopic infusion of seminal plasma (0.5 mL). There was no difference in intrauterine fluid accumulation between treatments in normal mares. HYST and SHAM treatments resulted in more fluid accumulation 24 hours after the procedures in DUC mares than UB and SP. However, leukocyte numbers were not different. It was concluded that the hysteroscopic procedure itself is inflammatory, so it should not be used with the intention of reducing post-mating endometritis. However, the inflammation was not greater than that induced by routine uterine body insemination and fertility was not affected. Therefore, there is no contraindication to its use in mares with delayed uterine clearance.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Ferrer_thesis.pdf 364.47 Kb 00:01:41 00:00:52 00:00:45 00:00:22 00:00:01

Browse All Available ETDs by ( Author | Department )

If you have questions or technical problems, please Contact LSU-ETD Support.